CUT&Tag for efficient epigenomic profiling of small samples and single cells

Hatice S. Kaya-Okur, Steven J. Wu, Christine A. Codomo, Erica S. Pledger, Terri D. Bryson, Jorja G. Henikoff, Kami Ahmad, Steven Henikoff

This paper describes CUT&Tag, an enzyme-tethering approach for highly efficient and sensitive epigenomic profiling from small samples and single cells. The method uses protein-A-Tn5 transposase fusion targeted by specific antibodies to perform tagmentation directly on chromatin in permeabilized cells or nuclei, eliminating the need for traditional ChIP-seq library preparation steps.